Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation. Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation

Cell lines have become an integral resource and tool for conducting biological experiments ever since the Hela cell line was first developed (1). They not only allow detailed investigation of molecular pathways but are faster and more cost-effective than most in vivo approaches. The last decade saw many emerging model systems strengthening basic science research. However, lack of genetic and molecular tools in these newer systems pose many obstacles. Astyanax mexicanus is proving to be an interesting new model system for understanding metabolic adaptation. To further enhance the utility of this system, we developed liver-derived cell lines from both surface-dwelling and cave-dwelling morphotypes. In this study, we provide detailed methodology of the derivation process along with a comprehensive biochemical and molecular characterization of the cell lines, which reflects key metabolic traits of cavefish adaptation. We anticipate these cell lines to become a useful resource for the Astyanax community as well as researchers investigating fish biology, comparative physiology, and metabolism. Overall design: 3 replicates each for 2 cell lines - SFL and CFL

自海拉细胞系 (Hela cell line) 首次建立以来，细胞系 (cell lines) 便已成为开展生物学实验不可或缺的核心资源与工具(1)。其不仅可用于对分子通路进行细致深入的研究，同时相较于绝大多数体内实验方法，具备更快的实验周期与更优的成本效益。近十年来，诸多新兴模型系统为基础科学研究注入了强劲动力，但这些新兴系统普遍缺乏遗传与分子研究工具，为相关研究带来了诸多阻碍。墨西哥丽脂鲤 (Astyanax mexicanus) 正逐渐成为解析代谢适应机制的极具潜力的新型模型系统。为进一步拓展该系统的应用价值，我们分别从表层栖息与洞穴栖息的形态型中成功构建了肝脏来源的细胞系。本研究详细阐述了该细胞系的构建流程，并对其开展了全面的生化与分子特征分析，这些分析结果精准反映了洞穴鱼适应环境的关键代谢特征。我们预期，这些细胞系将成为丽脂鲤研究领域，以及从事鱼类生物学、比较生理学与代谢研究的科研人员的实用研究资源。实验整体设计：本研究包含SFL与CFL两种细胞系，每种细胞系设置3个生物学重复。