Mouse bone marrow Mesenchymal Stem Cells (MSC) exposed to activated T cells

To analyze the changes induced in MSC transcriptome by activated T cells, MSC were co-cultured in transwell with anti CD3/CD28 activated T cells for 48 hrs before RNA extraction IFNγ-induced plasticity of mesenchymal stem cells is regulated by STATs through inhibition of mTOR and TGFβ pathways. Vigo T, Procaccini C, Ferrara G, Baranzini S, Oksenberg J, Diaspro A, Kerlero de Rosbo N, Uccella A. The expression of MSC co-cultured with activated T cells ( two replicates) were compared to that of naive MSC (3 replicates)

为探究活化T细胞对间充质干细胞（Mesenchymal Stem Cells, MSC）转录组的诱导改变，将MSC与经抗CD3/CD28抗体活化的T细胞在Transwell小室中共培养48小时，随后开展RNA提取实验。干扰素γ（Interferon γ, IFNγ）介导的间充质干细胞可塑性，可通过信号转导与转录激活因子（Signal Transducers and Activators of Transcription, STATs）抑制雷帕霉素靶蛋白（mammalian target of rapamycin, mTOR）与转化生长因子β（Transforming Growth Factor β, TGFβ）通路实现调控。该研究的作者包括Vigo T、Procaccini C、Ferrara G、Baranzini S、Oksenberg J、Diaspro A、Kerlero de Rosbo N及Uccella A。本实验将与活化T细胞共培养的MSC（2个生物学重复）的基因表达水平，与未活化的MSC（3个生物学重复）进行了对比分析。