Heterogeneous Cardiac- and Neural Crest-Derived Aortic Smooth Muscle Cells have Similar Transcriptional Changes after TGFβ Signaling Disruption [scRNA-seq-of-Nkx2-5-Cre-mTmG]. Heterogeneous Cardiac- and Neural Crest-Derived Aortic Smooth Muscle Cells have Similar Transcriptional Changes after TGFβ Signaling Disruption [scRNA-seq-of-Nkx2-5-Cre-mTmG]

We report single-cell RNA-seq data of Nkx2-5 lineage marked, integrin alpha 8 labeled smooth muscle cells (SMCs) from murine aortas at 12 weeks of age Overall design: Root/Ascending/Arch/Descending aortic segments were procured from 12-week-old Nkx2-5-Cre.mT/mG mice, ITGA8+ SMCs were isolated after cryophilic enzyme digestion, and single-cell RNA-seq analysis was performed

本数据集收录了12周龄小鼠主动脉中经Nkx2-5谱系标记、整合素α8（integrin alpha 8）标记的平滑肌细胞（SMCs）的单细胞RNA测序（single-cell RNA-seq）数据。实验设计：从12周龄Nkx2-5-Cre.mT/mG小鼠中获取主动脉根部、升主动脉、主动脉弓及降主动脉节段，经适冷酶消化后分离得到ITGA8+平滑肌细胞，随后开展单细胞RNA测序分析。