Expression and differential expression analysis of milk samples from healthy and diseased diary cows.. Bos taurus

Expression and differential expression analysis of milk samples from healthy and diseased diary cows. Diseases were grouped by their occurrence in the mammary gland or extra-mammary. Furthermore, the diseases were classified by their severity. All cows were examined thoroughly by the dairy herd manager, trained staff, or a veterinarian. Expression and differential expression was assessed by using the Affymetrix Bovine Genome Array (GPL2112). Control animals (2-4 years old, 1st to 3th lactation, one animal 4th and one 8th lactation) showed no clinical signs of disease and had no abnormalities in the udder or milk. Their somatic cell count (SCC) was less than 100,000 cells/ml milk. Most of the control samples were taken during early lactation (10-100 days post-partum, dpp). Diseased cows were in their 1st to 8th lactation within 10-220 dpp. Overall design: We analyzed 6 biological replicates each from milk samples from healthy diary cows, diary cows with mastitis and diary cows with endometritis.

健康与患病奶牛乳汁样本的基因表达及差异表达分析。本数据集按疾病发生于乳腺内或乳腺外对病症进行分组，此外还依据疾病严重程度对病症进行分级。所有受试奶牛均由牧场管理员、经过专业培训的工作人员或兽医完成全面检查。基因表达与差异表达分析采用Affymetrix牛基因组芯片（GPL2112）进行检测。对照组奶牛年龄为2~4岁，泌乳期为第1至3胎，另有1头处于第4泌乳期、1头处于第8泌乳期；该组奶牛无任何临床疾病症状，乳腺及乳汁均无异常，乳汁体细胞计数（Somatic Cell Count, SCC）低于100,000个/mL。多数对照样本采集于泌乳早期（产后10~100天，dpp）。患病奶牛均处于产后10~220天的泌乳阶段，泌乳期为第1至8泌乳期。实验整体设计：本研究分别采集健康奶牛、患乳腺炎奶牛及患子宫内膜炎奶牛的乳汁样本，每组均设置6个生物学重复并开展分析。