Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the Drosophila mushroom body contributing to olfactory learning

O-GlcNAcylation is a dynamic post-translational modification that diversifies the proteome. Its dysregulation is associated with neurological disorders that impair cognitive function, and yet identification of phenotype-relevant candidate substrates in a brain-region-specific manner remains unfeasible. By combining an O-GlcNAc binding activity derived from Clostridium perfringens OGA (CpOGA) with TurboID proximity labeling in Drosophila, we developed an O-GlcNAcylation profiling tool that translates O-GlcNAc modification into biotin conjugation for tissue-specific candidate substrates enrichment. We mapped the O-GlcNAc interactome in major brain regions of Drosophila and found that components of the translational machinery, particularly ribosomal subunits, were abundantly O-GlcNAcylated in the mushroom body of Drosophila brain. Hypo-O-GlcNAcylation induced by ectopic expression of active CpOGA in the mushroom body decreased local translational activity, leading to olfactory learning def..., , , # Data from: Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the *Drosophila* mushroom body contributing to olfactory learning

DOI:[10.5061/dryad.sj3tx969t ](https://doi.org/10.5061/dryad.sj3tx969t)

This dataset includes the data in the above publication. In this publication, we developed an *O*-GlcNAcylation profiling tool that translates *O*-GlcNAc modification into biotin conjugation for tissue-specific candidate substrate enrichment. We mapped the *O*-GlcNAc interactome in major brain regions of *Drosophila* and found that components of the translational machinery, particularly ribosomal subunits, were abundantly *O*-GlcNAcylated in the mushroom body of ...

O-连接N-乙酰葡糖胺糖基化（O-GlcNAcylation）是一类可赋予蛋白质组多样性的动态翻译后修饰（post-translational modification）。该修饰的失调与损害认知功能的神经系统疾病密切相关，但以脑区特异性方式鉴定表型相关候选底物仍难以实现。我们将源自产气荚膜梭菌（Clostridium perfringens）OGA（CpOGA）的O-GlcNAc结合活性，与果蝇（Drosophila）中的TurboID邻近标记技术相结合，开发了一款O-连接N-乙酰葡糖胺糖基化分析工具，可将O-GlcNAc修饰转化为生物素缀合反应，从而实现组织特异性候选底物的富集。我们绘制了果蝇主要脑区的O-GlcNAc相互作用组图谱，并发现翻译机器的组分，尤其是核糖体亚基，在果蝇脑的蕈形体（mushroom body）中存在大量O-GlcNAc修饰。在蕈形体中异位表达活性CpOGA所诱导的O-连接N-乙酰葡糖胺糖基化不足，会降低局部翻译活性，进而导致嗅觉学习缺陷……

# 数据源自：《组织特异性O-连接N-乙酰葡糖胺糖基化分析鉴定果蝇蕈形体翻译机器底物并参与嗅觉学习》
DOI: 10.5061/dryad.sj3tx969t

本数据集包含上述发表论文中的相关数据。在该研究中，我们开发了O-连接N-乙酰葡糖胺糖基化分析工具，可将O-GlcNAc修饰转化为生物素缀合反应以实现组织特异性候选底物富集。我们绘制了果蝇主要脑区的O-GlcNAc相互作用组图谱，并发现翻译机器组分（尤其是核糖体亚基）在果蝇脑蕈形体中大量发生O-GlcNAc修饰……