Diversity and Community Structure of Primary Wood-Inhabiting Bacteria in Boreal Forest

DNA-based pyrosequencing analysis of the V1- V3 16S rRNA gene region was used to identify bacteria community and shift during early stages of wood colonization in boreal forest soils. The dataset comprised 142,447 sequences and was affiliated to 11 bacteria phyla, 25 classes and 233 genera. The dominant groups across all samples were <i>Proteobacteria</i>, followed by <i>Bacteroidetes</i>, <i>Acidobacteria</i>, <i>Actinobacteria</i>, <i>Amatimonadetes</i>, <i>Planctomycetes</i> and TM7 group. The community structure of the primary wood-inhabiting bacteria differed between types of forest soils and the composition of bacteria remained stable over prolonged incubation time. The results suggest that variations in soil bacterial community composition have an influence on the wood-inhabiting bacterial structure.

本研究通过针对V1-V3区16S rRNA基因的DNA焦磷酸测序（pyrosequencing）分析，解析了北方森林土壤中木材定殖早期阶段的细菌群落组成及其动态变化。本数据集共包含142447条序列，可归类至11个细菌门、25个细菌纲与233个细菌属。所有样本中的优势类群依次为<i>变形菌门（Proteobacteria）</i>、<i>拟杆菌门（Bacteroidetes）</i>、<i>酸杆菌门（Acidobacteria）</i>、<i>放线菌门（Actinobacteria）</i>、<i>阿米巴菌门（Amatimonadetes）</i>、<i>浮霉菌门（Planctomycetes）</i>以及TM7类群。原始木材定殖细菌的群落结构因森林土壤类型不同而存在显著差异，且细菌群落组成在长期培养过程中保持相对稳定。研究结果表明，土壤细菌群落组成的差异会对木材定殖细菌的群落结构产生影响。