Single-cell and bulk RNA sequencing of mouse atherosclerosis disease stage course [scRNASeq_WAT_Mono]

Atherosclerotic coronary artery disease (CAD) development encompasses changes in immune cell activation across different tissues, including white adipose tissue (WAT). However, the specific disease-associated transcriptional changes occurring within each of the tissue cell types remain incompletely characterized. Here, we provide a scRNA-seq dataset allowing the characterization of disease-associated cell states within the major WAT cell types. For the myeloid cells, we further analyzed sub-populations and characterized their protein-coding and miRNA gene markers. By comparing to naive monocytes isolated from blood, we further used the tissue profiles to interrogate how the tissue infiltration changes the monocyte and dendritic cell transcriptome profiles. Overall design: A disease stage course of atherosclerosis in mouse was established by varying genotype (wild type and proatherogenic) and diet (0, 1 or 3 months of high-fat diet), followed by scRNA-Seq (all cell types) and bulk RNA-Seq of the thoracic aorta.

动脉粥样硬化性冠状动脉疾病（CAD）的发生发展，涵盖包括白色脂肪组织（WAT）在内的多种组织内免疫细胞活化状态的改变。然而，目前尚未完全阐明每种组织细胞类型中与疾病相关的特异性转录组变化。本研究提供一套单细胞RNA测序（scRNA-seq）数据集，可用于解析白色脂肪组织主要细胞类型内与疾病相关的细胞状态。针对髓系细胞，本研究进一步分析了其亚群，并鉴定了其蛋白编码基因与微小RNA（miRNA）的基因标记物。通过与血液中分离的初始单核细胞进行对照，本研究进一步利用组织转录组图谱，探究组织浸润如何改变单核细胞与树突状细胞的转录组特征。整体实验设计：本研究通过调整小鼠基因型（野生型与致动脉粥样硬化型）与饮食方案（普通饮食，或高脂饮食喂养0、1、3个月）构建动脉粥样硬化疾病进程模型，随后对所有细胞类型开展单细胞RNA测序（scRNA-seq），并对胸主动脉进行批量RNA测序（bulk RNA-seq）。