Characterization of H3K4me3 histone modifications in intestinal epithelial cells among healthy controls and newly-diagnosed pediatric Crohn's disease patients.. Characterization of H3K4me3 histone modifications in intestinal epithelial cells among healthy controls and newly-diagnosed pediatric Crohn's disease patients.

We report profiling of H3Kme3 histone modifications in intestinal epithelial cells from ileal endoscopic biopsies obtained from healthy controls and newly-diagnosed Crohn's disease (CD). We identified 1066 shared sites, corresponding to 1038 genes with increased H3K4me3 in CD, and 539 sites corresponding to 548 genes with reduced H3K4me3 in CD. Overall design: We examine chromatin H3Kme3 modifications through ChIP-seq of intestinal epithelial cells harvested from ileal endoscopic biopsies obtained from healthy controls and newly-diagnosed Crohn's patients.

本研究报道了健康对照及新诊断克罗恩病（Crohn's disease, CD）患者回肠内镜活检标本来源的肠上皮细胞中H3Kme3组蛋白修饰的谱分析结果。本研究共鉴定出1066个共有修饰位点，其中对应CD患者中H3K4me3水平升高的基因共1038个，另有539个修饰位点对应CD患者中H3K4me3水平降低的548个基因。整体实验设计：本研究采用染色质免疫共沉淀测序（ChIP-seq）技术，对从健康对照及新诊断克罗恩病患者的回肠内镜活检标本中分离得到的肠上皮细胞的染色质H3Kme3修饰进行检测分析。