The transcriptomic response of skeletal muscle in life-long high-level trained and untrained men and women after acute exercise

To investigate the influence of lifelong exercise training on the response of skeletal muscle to a bout of acute exercise we generated global transcriptomic data from long-term endurance (8 men, 8 women) and strength (8 men, 8 women) trained individuals and healthy age-matched untrained controls (8 men, 8 women). Skeletal muscle biopsies were taken from M. vastus lateralis before, directly after, and after 1h and 3hrs following acute exercise. All subjects completed one bout of acute endurance exercise and one bout of acute resistance exercise, separated by 4-8 weeks. All 384 samples were multiplexed in 4 lanes and sequenced (2x250bp paired end) on the Illumina NovaSeq 6000.EGA study EGAS00001006139

为探究终身运动训练对骨骼肌单次急性运动应答的影响，我们从长期耐力训练（8名男性、8名女性）、力量训练（8名男性、8名女性）个体，以及年龄匹配的健康未训练对照（8名男性、8名女性）中生成了全局转录组数据。分别于急性运动前、运动即刻以及运动后1小时、3小时，从股外侧肌（M. vastus lateralis）采集骨骼肌活检样本。所有受试者均完成一次急性耐力运动与一次急性抗阻运动，两次运动间隔4至8周。全部384个样本在4个测序泳道中完成多重复合，并依托Illumina NovaSeq 6000平台开展2×250bp双端测序。本研究的欧洲基因组档案（EGA）编号为EGAS00001006139。