Evolutionary and functional analysis of DNA methyltransferases in micro-eukaryotes: Insights from the model diatom Phaeodactylum tricornutum [Bisulfite-Seq]

DNA methylation has been associated with transcriptional repression of transposable elements. Here we describe the first DNA methylation deficient cell line of a diatom by generating DNMT5:KOs in Phaeodactylum tricornutum. These data show the extend of the loss of DNA methylation in DNMT5:KOs compared to the reference strain. All DNA methylation is lost in DNMT5:KOs. Overall design: 3 conditions - 2 independent DNMT5:KOs -> DNMT5:KO line M23 ; DNMT5:KO line M25 + 1 reference strain "WT" Pt18.6. 1 replicate per condition.

DNA甲基化（DNA methylation）已被证实与转座因子（transposable elements）的转录抑制相关。
本研究通过在三角褐指藻（Phaeodactylum tricornutum）中构建DNMT5基因敲除株系（DNMT5:KOs），首次获得了硅藻的DNA甲基化缺陷细胞系。
本数据集揭示了DNMT5基因敲除株系与参考菌株相比的DNA甲基化丢失程度。
DNMT5基因敲除株系中完全丧失了DNA甲基化。
实验设计概况：共设置3组实验条件——2株独立的DNMT5基因敲除株系：DNMT5:KO株系M23、DNMT5:KO株系M25，以及1株参考野生型菌株"WT Pt18.6"；每组条件设置1次重复。