An integrated network pharmacology and RNA-seq approach for exploring the vascular protection of neferine on attenuating Ang II-induced blood pressure elevation

The goals of this study is to compare the differently expressed genes in abdominal aorta tissues of Control and Ang II with or without neferine treatment. The Ang II (n=12) were randomly divided into 2 groups: Ang II , and Ang II + neferine (1 mg/kg/day) groups (n=6 for each group). Control groups (n=5) were set as control. Mouse in Control and Ang II groups were intragastrically with double distilled water (dd H2O); while mice in Ang II + neferine (1 mg/kg/day) group were intragastrically with 1 mg/kg/day of neferine for 6 weeks. Then the abdominal aorta tissues were used to identify differentially expressed genes among different groups. Overall design: RNA sequencing was performed with abdominal aorta of mice in 3 groups:Control(n=5), Ang II(n=6 ) , Ang II + neferine(1 mg/kg/day,n=6) treated to identify the differently expressed genes and related pathway.

本研究旨在比较对照组、单纯血管紧张素II（Angiotensin II, Ang II）处理组及联合莲心碱（neferine）处理组小鼠腹主动脉组织中的差异表达基因。将血管紧张素II造模小鼠（n=12）随机分为2组：单纯Ang II处理组与Ang II+莲心碱（1 mg/kg/天）处理组，每组n=6；另设空白对照组（n=5）。空白对照组与单纯Ang II处理组小鼠均以双蒸水（double distilled water, ddH₂O）灌胃，Ang II+莲心碱处理组小鼠则以1 mg/kg/天的莲心碱灌胃，持续干预6周。随后采集各组小鼠腹主动脉组织，用于鉴定不同组别间的差异表达基因。整体实验设计：本研究对3组小鼠的腹主动脉组织开展RNA测序（RNA sequencing），即空白对照组（n=5）、单纯Ang II处理组（n=6）及Ang II+莲心碱（1 mg/kg/天，n=6）处理组，以筛选差异表达基因并解析其相关调控通路。