Hematopoietic Progenitor Cells of Different Donor Age. Homo sapiens

In this series we have analyzed the effect of donor age on the gene expression profile of human hematopoietic stem and progenitor cells (HPC). Cells were taken from umbilical cord blood (CB) or from G-CSF mobilized blood of healthy donors for allogeneic blood stem cell transplantation. Overall design: Hematopoietic progenitor cells (HPC) were isolated within the CD34+ cell fraction from fresh human cord blood (CB) or from G-CSF mobilized peripheral blood (PB) as described in detail before (Wagner et al., Blood, 2004, 104:675-684; Wagner et al., Stem cells, 2005, 23:1180-1191; Wagner et al., 2007, 10:2638-2657). Differential gene expression was subsequently compared in the CD34+ samples of different donor age.

本系列研究分析了供者年龄对人类造血干细胞与祖细胞（hematopoietic stem and progenitor cells，HPC）基因表达谱的影响。实验所用细胞取自用于异基因造血干细胞移植的健康供者的脐带血（CB）或粒细胞集落刺激因子（granulocyte colony-stimulating factor，G-CSF）动员的血液。实验整体设计如下：如既往详细报道所述（Wagner等，《血液》，2004，104：675-684；Wagner等，《干细胞》，2005，23：1180-1191；Wagner等，2007，10：2638-2657），研究人员从新鲜人类脐带血（CB）或粒细胞集落刺激因子动员的外周血（peripheral blood，PB）的CD34+细胞组分中分离得到造血祖细胞（HPC）。随后对不同供者年龄的CD34+样本进行差异基因表达比较。