Regulation of RNA translation among human primary immune cells

We performed RNase footprinting and RNA-seq to examine the regulation of RNA translation comparing human primary immune cell types, including T-cells, B-cells, monocytes. RNase footprinting and RNA-seq of T-cells, B-cells, monocytes, and Peripheral blood mononuclear cells (PBMCs).本研究采用核糖核酸酶足迹法（RNase footprinting）与RNA测序（RNA-seq），针对包含T细胞、B细胞、单核细胞在内的人类原代免疫细胞类型，探究RNA翻译调控机制并开展组间比较分析。此外，本研究还对T细胞、B细胞、单核细胞及外周血单个核细胞（PBMCs）实施了核糖核酸酶足迹法与RNA测序实验。