Estrogen Drives Melanocortin Signaling to Increase Spontaneous Activity

Transcriptional profilig of estradiol sensitive genes in the VMHvl of female mice Overall design: For the vehicle treatment condtion, 3 biological replicates were analyzed. In the estradiol benzoated (EB) treated group, 4 biological replicates were analyzed. In both cases, each biological replicate represents material isolated from a single mouse. Please note that the mice are on a mixed background (as they are originally derived from the floxed Esr1 line generated in Sohaib Khan's lab, PMID: 17785410, and therefore come from a 129/Sv lineage).

雌性小鼠下丘脑腹内侧核腹外侧部（VMHvl）内雌二醇敏感基因的转录谱分析（transcriptional profiling）

整体实验设计：溶剂处理组设置3个生物学重复（biological replicates）进行分析；苯甲酸雌二醇（estradiol benzoated, EB）处理组设置4个生物学重复进行分析。两组的每个生物学重复均取自单只小鼠的分离实验材料。请注意，本实验所用小鼠为混合遗传背景：其最初源自Sohaib Khan实验室构建的Esr1基因floxed小鼠品系（PMID: 17785410），因此起源于129/Sv小鼠谱系。