Impact of Surfactant protein B (SP-B) on high density lipoprotein (HDL) oxidation index

Circulating immature surfactant protein B (proSP-B) forms emerged as the most reliable lung-specific circulating marker for alveolar-capillary membrane (ACM) dysfunction and for the overall clinical status of heart failure. Of note, ProSP-B forms were detectable in high-density lipoprotein (HDL) only. Thus we assessed the impact of proSP-B on HDL functions using HDL from healthy subjects enriched with proSP-B produced by a stably transfected CHO cell line that specifically expresses and releases the human proSP-B. In particular, HDL antioxidant capacity (HOI) has been assessed in terms of ability to inhibit air-induced LDL oxidation. Data from this dataset demonstrated that HOI was impaired after proSP-B enrichment (Table1). HOI was also higher in HF patients with respect to age-matched control healthy subjects (Table2).

循环型未成熟表面活性蛋白B（proSP-B）已被证实为反映肺泡毛细血管膜（ACM）功能障碍以及心力衰竭整体临床状态的最可靠肺特异性循环标志物。值得注意的是，proSP-B仅可在高密度脂蛋白（HDL）中被检测到。因此，本研究通过采用稳定转染并特异性表达、分泌人源proSP-B的中国仓鼠卵巢（CHO）细胞系所制备的proSP-B，对健康受试者的HDL进行富集，以此评估proSP-B对HDL功能的影响。具体而言，本研究以HDL抑制空气诱导的低密度脂蛋白（LDL）氧化的能力为指标，对其抗氧化能力（HOI）进行了评估。本数据集的数据证实，经proSP-B富集后，HDL的抗氧化能力（HOI）出现受损（表1）。相较于年龄匹配的健康对照受试者，心力衰竭（HF）患者的HDL抗氧化能力（HOI）水平更高（表2）