Fluorescence in situ hybridization (FISH) images of methanotrophic (MOX, MITC849 probe double-labelled with 594 dye) symbionts in 10 μm sections of H. (S.) methanophila sponge (Mictlan Knoll individual).

Twenty 3-channel images were acquired with Zeiss Axioplan 2 epifluorescence microscope (Zeiss, Jena, Germany) with 10x magnification. Color legend is: MOX, magenta; DNA (DAPI), blue; autofluoresence at ~520 nm with ~490 nm excitation (FITC filter), green. These images were aligned with FIJI Grid/collection stitching plugin to produce the resulting mosaic.本数据集包含20张三通道图像，均由德国耶拿蔡司（Zeiss）公司的Axioplan 2型落射荧光显微镜（epifluorescence microscope）以10倍放大倍率采集。图像颜色图例如下：MOX标记为品红色；DNA（4',6-二脒基-2-苯基吲哚，DAPI）标记为蓝色；以约490nm光激发、对应~520nm波段的自发荧光（采用FITC滤光片）标记为绿色。随后使用FIJI软件的Grid/Collection拼接插件对上述图像进行配准拼接，得到最终的拼接镶嵌图像。