cisplatin-damaged gene locus-replicate 2-Blank-R1

Cisplatin is a DNA-targeting chemotherapeutic, yet its damaged gene loci are largely unexplored. We utilized a forward chemical genetics strategy to map 7578 cisplatin-damaged genes (CDGs) with >12 fold-enrichment from A549 human lung cancer cells, of which the highly associated signalling pathways include sperm motility, molecular mechanism of cancer, and protein kinase A signalling. The three most frequently cisplatinated penta-nucleotide motifs and genomic region are CTGGG, CCAGG and GCTGG, and promoter, respectively. Among the CDGs are 1330 enzyme, 747 transcription regulator and 486 transporter genes. Importantly, cisplatin targeted 306 protein kinase genes, accounting for 59% of putative protein kinase genes in the human genome, and 92 protein phosphatase genes. This indicates that cisplatin genome-wide disturbs protein phosphorylation. This data set contains the original sequencing data of two biological replicates, matching results to the hg38 genome, and the analysis results of the distribution of the damage areas identified by the two sequencing results.

顺铂（cisplatin）是一类靶向DNA的化疗药物，但其诱导产生DNA损伤的基因位点仍未得到充分探究。本研究采用正向化学遗传学策略，从人肺癌A549细胞中筛选并定位得到7578个富集倍数>12的顺铂损伤基因（cisplatin-damaged genes, CDGs）；其中显著富集的信号通路包括精子运动、癌症分子机制及蛋白激酶A（protein kinase A）信号通路。最频繁被顺铂修饰的五核苷酸基序依次为CTGGG、CCAGG与GCTGG，而最易发生顺铂损伤的基因组区域为启动子区域。在上述CDGs中，包含1330个酶编码基因、747个转录调控因子编码基因及486个转运蛋白编码基因。值得注意的是，顺铂共靶向306个蛋白激酶基因，占人类基因组中推定蛋白激酶基因总数的59%，同时还靶向了92个蛋白磷酸酶基因。这表明顺铂可在全基因组范围内干扰蛋白质磷酸化过程。本数据集包含两份生物学重复的原始测序数据、与hg38人类参考基因组的比对结果，以及基于两份测序结果鉴定得到的损伤区域分布分析结果。