MYCL and EP400 are required for Max and MCPyV mediated gene activation

To determine if MYCL or EP400 knockdown would affect Max target genes in Merkel cell carcinoma cell line MKL-1, we performed RNA-seq analyses of MKL-1 cells inducibly expressing shMYCL and two different EP400 shRNA -2, -3 and compared to ChIP-seq data using BETA analyses. MYCL or EP400 was inducibly reduced with shRNA in MKL-1 cells to analyze gene regulation.

为探究敲低MYCL或EP400是否会影响默克尔细胞癌（Merkel cell carcinoma）细胞系MKL-1中的Max靶基因，我们对诱导表达靶向MYCL的短发夹RNA（shRNA），以及两种分别为shRNA-2、shRNA-3的EP400靶向短发夹RNA的MKL-1细胞开展了RNA测序（RNA-seq）分析，并采用BETA分析（BETA analyses）将所得结果与染色质免疫共沉淀测序（ChIP-seq）数据进行比对。本研究通过短发夹RNA在MKL-1细胞中诱导下调MYCL或EP400的表达水平，以此分析二者对基因调控的影响。