Bacterial diversity data supporting study of changes in Louisiana marsh microbial communities after the Deepwater Horizon oil spill, May 2010-September 2011, August 2013

This study was done to evaluate changes in indigenous microbial communities from coastal marsh sediments after the Gulf of Mexico oil spill in 2010. This study was conducted from eleven coastal marshes in southern Louisiana. Samples were acquired from May 2010 through August 2013, focusing on sediments approximately 1 m offshore from the edge of the marsh. Marshes were oiled from late June-August 2010, and some marshes were reoiled in 2011 from tropical storms and hurricanes. Some marshes close to the Mississippi River were impacted by freshwater diversions in 2010. Bacterial communities were assessed from sediments at 0-1 cm and 1-2 cm depth intervals. The results are amplicon pyrosequence libraries of single-direction reads generated using multiplexed, bar-coded primers targeting the V1-V3 region of the bacterial of 16S rRNA gene.

本研究旨在评估2010年墨西哥湾漏油事件后，沿海沼泽沉积物中土著微生物群落的变化。本研究选取路易斯安那州南部的11处沿海沼泽开展采样工作，样本采集时间为2010年5月至2013年8月，采样点位聚焦于沼泽边缘向海一侧约1米处的沉积物。2010年6月下旬至8月，部分沼泽受到原油污染；2011年，部分沼泽因热带风暴和飓风引发二次溢油。另有部分紧邻密西西比河的沼泽在2010年受到淡水分流工程的影响。研究分别采集0-1 cm及1-2 cm深度的沉积物样本以解析细菌群落组成。本研究的测序结果为包含单端测序读段（single-direction reads）的扩增子焦磷酸测序文库（amplicon pyrosequence libraries），该文库采用多重条形码标记引物，靶向细菌16S核糖体RNA基因（16S rRNA gene）的V1-V3可变区构建得到。