Comparative Transcriptomic Analysis of Dermal Wound Healing Reveals De Novo Skeletal Muscle Regeneration in Acomys cahirinus

This project is a comprehensive analysis of gene expression following full-thickness excisional dermal wounds in the African spiny mouse and the laboratory mouse. 8mm excisional wounds were made on the dorsal skin. Wounds were harvested on day 7 and 14 after wounding. Normal, unwounded (day 0) skin was used as a control. N=4 for each time point for each species. 2x150 bp paired-end reads. DSN library was equal amounts of all Acomys samples, pooled, and normalized using duplex-specific nuclease. The 'Mus_Day-0_rep3' sample was removed from downstream analysis due to poor QC results, and is not included here.

本项目针对非洲刺毛鼠（Acomys）与实验室小鼠的全层切除性皮肤创伤后的基因表达情况开展了系统性分析。研究人员在两类小鼠的背部皮肤制作直径8mm的全层切除创口，分别于创伤后第7天和第14天采集创口样本；以未受创伤的正常皮肤（创伤后第0天）作为对照。每个物种的每个时间点均设置4个生物学重复（N=4），测序采用2×150 bp双端测序读段（paired-end reads）。针对所有非洲刺毛鼠样本，取等量混合后采用双链特异性核酸酶（duplex-specific nuclease，DSN）进行文库均一化处理，构建DSN文库。由于质量控制（QC）结果不佳，"Mus_Day-0_rep3"样本被移除于后续分析之外，未纳入本数据集。