Regulation of Endogenous Retroviruses and Cellular Genes by HIV-1 Rev II

A combination of bioinformatics, RNAseq, and cell-based functional assays were used to study the changes in host cell gene expression when HIV-1 Rev or Nef was expressed. These results suggest that HIV Rev and Nef are capable of modulating gene and mRNA isoform expression, during HIV-1 infection. Overall design: Investigation into host cell gene expression when HIV-1 Rev or HIV-1 Nef is expressed in T-lymphocyte cells.本研究联合应用生物信息学（bioinformatics）、RNA测序（RNAseq）及基于细胞的功能检测（cell-based functional assays），对表达HIV-1 Rev或Nef时宿主细胞的基因表达变化进行了探究。结果显示，在HIV-1感染进程中，HIV Rev与Nef能够调控基因及mRNA剪接异构体的表达。 整体实验设计：在T淋巴细胞中表达HIV-1 Rev或HIV-1 Nef，以此探究宿主细胞的基因表达变化。