Acute Kidney Injury Transcriptional Profiles: Single Cell RNA Sequencing reveals Injury induced Renal Developmental program reactivation and Lineage Infidelity in adult kidney. Acute Kidney Injury Transcriptional Profiles: Single Cell RNA Sequencing reveals Injury induced Renal Developmental program reactivation and Lineage Infidelity in adult kidney

Acute Kidney Injury (AKI) is a rapid renal function decline associated with pronounced morbidity and mortality. Single Cell RNA Sequencing is a powerful tool allowing for examining transcriptional changes in multiple renal cell populations involved in the injury response. Our study reveals renal developmental gene re-activation and lineage infidelity in response to ischemia/reperfusion induced AKI, along with the novel genes which might serve as markers of acute kidney disease Overall design: Using droplet RNA sequencing, transcriptomes of single cells from normal and injured mouse kidneys were analyzed

急性肾损伤（Acute Kidney Injury，AKI）是一类伴随显著发病率与死亡率的快速肾功能减退病症。单细胞RNA测序（Single Cell RNA Sequencing）是一种强大的研究工具，可用于探究损伤应答过程中多种肾细胞群体的转录组变化。本研究揭示了缺血再灌注诱导的AKI中，肾脏发育相关基因发生重激活以及细胞谱系保真度丧失的现象，同时还发现了潜在可作为急性肾脏疾病标志物的新型基因。实验整体设计：采用液滴RNA测序技术，对正常与损伤小鼠肾脏的单细胞转录组进行了分析。