Temperature effects on the development and metamorphosis of larvae of broadcast spawning coral species from Japan and the Great Barrier Reef

In August 2004, an experiment was conducted to test the effects of temperature on larval development and metamorphosis for the solitary coral, Fungia repanda, collected from a patch reef slope in northern Okinawa, Japan. Three hours after fertilisation, the embryos from a single male and female colony cross were transferred into triplicate 2 litre plastic beakers, maintained in incubators set at 26, 28 and 30°C (±0.5°C).Larvae were sampled from each beaker on days 3 and 4 post-spawning and transferred to six-well cell culture plates (8-12 larvae per well, n = 3 wells) containing 10 ml of 1 µm filtered sea water (FSW) and a small (

2004年8月，本研究开展了一项实验，以探究温度对单体珊瑚蕈珊瑚（Fungia repanda）幼体发育与变态的影响，该珊瑚采集自日本冲绳北部的斑块礁斜坡。受精3小时后，将单个雌雄群体杂交获得的胚胎转移至3组重复的2升塑料烧杯中，所有烧杯均置于温度设定为26、28和30°C（误差±0.5°C）的培养箱内培养。在产卵后第3天和第4天，从每个烧杯中采集幼体，转移至六孔细胞培养板中（每孔8~12个幼体，共3个孔），培养板内含有10毫升1微米过滤海水（FSW）及少量（