RNA-seq Transcriptome Analysis of AD169 and AD169-ΔUL26 infected MRC5 fibroblasts.. RNA-seq Transcriptome Analysis of AD169 and AD169-ΔUL26 infected MRC5 fibroblasts.

We employed RNA-seq to map the transcriptome of human MRC5 fibroblasts during HCMV infection with AD169 and AD169-ΔUL26 strains. These data will highlight the ways in which the HCMV UL26 protein alters host gene transcripton during infection. Overall design: Infect quiescent MRC5 fibroblasts with HCMV AD169 and AD169-ΔUL26 strains, in 15-cm dishes at MOI = 3 and harvest samples for RNA-seq at 48 hpi.

本研究采用RNA测序（RNA-seq）技术，绘制了人类巨细胞病毒（Human Cytomegalovirus, HCMV）AD169株及AD169-ΔUL26株感染人MRC5成纤维细胞过程中的转录组图谱。本数据集将阐明HCMV UL26蛋白在感染进程中调控宿主基因转录的具体途径。总体实验设计：将处于静息态的MRC5成纤维细胞以感染复数（Multiplicity of Infection, MOI）=3接种于15cm培养皿中，分别用HCMV AD169株与AD169-ΔUL26株进行感染，并于感染后48小时（hours post infection, hpi）收集样本用于RNA测序。