RNA changes in the cortex of 1-month old ICR mice overexpressing microRNA142-GFP compared to controls overexpressing tdtomato in the cortical neurons as determined by mRNA deep sequencing.

The overall goal of the project is to assess the mRNA alterations in the cortex of mice overexpressing microRNA142 (miR142). The wildtype ICR mice were electroporated with miR142-GFP plasmid DNA construct and tdtomato plasmid DNA control construct at embryonic day 14.5 (E14.5) using the technique in-utero electroporation (IUE). At this age, only cortical neurons get electroporated. At one-month of age, the cortices of these mice were evaluated for the global expression of mRNAs by mRNA deep sequencing. The levels of several mRNAs were altered. Ingenuity pathway network analysis (IPA) was performed on differentially altered mRNAs. Overall design: Five samples per each plasmid construct.本项目的整体目标是评估过表达微小RNA142（microRNA142, miR142）的小鼠大脑皮层内的mRNA表达变化。研究人员于胚胎第14.5天（E14.5），采用子宫内电穿孔（in-utero electroporation, IUE）技术，向野生型ICR小鼠体内分别电转染miR142-GFP质粒DNA构建体与作为对照的tdTomato质粒DNA构建体；该发育阶段仅大脑皮层神经元会被电转染。待小鼠生长至1月龄时，通过mRNA深度测序对其大脑皮层的mRNA整体表达水平进行检测，结果显示多条mRNA的表达水平发生改变。研究人员对差异表达的mRNA开展了Ingenuity通路网络分析（Ingenuity pathway network analysis, IPA）。实验整体设计：每组质粒构建体对应5个样本。