Single cell ATAC sequencing profile of quiescent mesenchymal progenitors

After tissue injury mesenchymal progenitor subsets undergo a transient proliferative expansion, morphological transition and migration from their perivascular niche. Some of these subsets contribute enduring populations to connective tissue structures adjacent to muscle. To gain insights into gene accessibility to complement the single cell RNA seq expression data previously obtained and to uncover the lineage potential of MPs in this context, tdTomato positive cells were profiled by single cell ATAC sequencing. Overall design: Single cell ATAC-seq analyses of enriched quiescent mesenchymal progenitors from skeletal muscle.

组织损伤后，间充质祖细胞亚群（mesenchymal progenitor subsets）会经历暂时性增殖扩增、形态学转变，并从其血管周围龛位（perivascular niche）迁出。其中部分亚群会为肌肉相邻的结缔组织结构提供持久存在的细胞群体。为了探究基因可及性以补充此前获得的单细胞RNA测序（single-cell RNA sequencing）表达数据，并阐明该背景下间充质祖细胞（MPs, Mesenchymal Progenitors）的谱系潜能，研究人员通过单细胞ATAC测序（single-cell Assay for Transposase-Accessible Chromatin sequencing, scATAC-seq）对tdTomato阳性细胞进行了特征分析。整体实验设计：对源自骨骼肌的富集静止态间充质祖细胞开展单细胞ATAC-seq分析。