Chromosomal rearrangements juxtapose the TERT coding sequence to strong enhancer elements

Chromatin immunoprecipitation (ChIP) sequencing with an anti-acetyl-H3K27 antibody showed an enhancer region rearranged to the TERT gene, and ChIP sequencing with an anti-BRD4 antibody showed BRD4 protein binding to the enhancer in TERT-rarranged neuroblasstoma PDX CCI-NB07-RMT cells Examination of H3K27ac signal and BRD4 binding in TERT-rearranged neuroblastoma PDX cells

使用抗乙酰化H3K27抗体（anti-acetyl-H3K27 antibody）的染色质免疫沉淀（ChIP）测序结果显示，一段增强子区域发生重排并衔接至端粒酶逆转录酶（TERT）基因；采用抗BRD4抗体（anti-BRD4 antibody）的染色质免疫沉淀测序则证实，在TERT重排型神经母细胞瘤（neuroblastoma）患者来源异种移植（Patient-Derived Xenograft, PDX）CCI-NB07-RMT细胞中，BRD4蛋白（BRD4 protein）可结合至该重排增强子区域。本数据集涵盖针对TERT重排型神经母细胞瘤PDX细胞中H3K27ac信号与BRD4结合情况的检测分析。