Example dataset for myofiber type composition analysis

Here, we present a small subset of human and mouse datasets that were immunostained with monoclonal antibodies to the three most abundant MyHC isoforms (in human: MyHC1, MyHC2A and MyHC2X; in mouse, MyHC2X was replaced with MyHC2B) and laminin. These images can be used to run our step-by-step semiautomated approach for quantitative myofiber type composition analysis (https://github.com/tabbassidaloii/ImageProcessing/tree/main/MyofiberTyping).

本研究提供了一套小型的人类与小鼠数据集子集，该数据集使用针对三种丰度最高的肌球蛋白重链（MyHC）亚型的单克隆抗体以及层粘连蛋白（laminin）进行免疫染色。其中，人类样本对应的三种亚型为MyHC1、MyHC2A与MyHC2X；小鼠样本中则将MyHC2X替换为MyHC2B。这些图像可用于运行我们的分步半自动分析流程，以开展定量肌纤维分型组成分析（代码仓库地址：https://github.com/tabbassidaloii/ImageProcessing/tree/main/MyofiberTyping）。