SH1043314.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、传播以及处理基于DNA的物种假说（Species Hypotheses，SH）。国际核苷酸序列数据库中的所有真菌内部转录间隔区（Internal Transcribed Spacer，ITS）序列，将通过应用一组动态距离阈值（0.5% - 3.0%）被聚类至近似物种水平的簇群。所有物种假说（SH）都会被赋予唯一且稳定的数字对象标识符（Digital Object Identifier，DOI）格式的专属名称，其分类学与生态学注释信息将通过分布式的网页端第三方注释工作完成校验。每个SH都会尽可能匹配对应的分类单元名称及其所属的分类层级（如门、纲、目等），匹配依据为该SH下所有序列的鉴定结果。随后会选取一条自动或人工指定的序列作为每个SH的代表序列。这些代表序列已发布于https://unite.ut.ee/repository.php，可供科学界用于本地序列相似性搜索、下一代测序分析流程等科研场景。随着公共真菌ITS序列库的规模不断增长，该系统及其配套数据会自动完成更新。