Transcriptome comparison of PMN-MDSCs and and normal neutrophils

Polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC), also named pathologically activated neutrophil, is a critical component of tumor microenvironment (TME), playing crucial roles in tumor progression and therapy resistance. Here, we compared the transcriptome of PMN-MDSCs from B16 tumor bearing mice and the neutrophils from tumor free mice. Overall design: B16-F10 melanoma cells were inoculated subcutaneously into wild-type C57/BL6 mice. At day 15 post inoculation, PMN-MDSCs were isolated spleen of tumor bearing (TB) mice by AutoMACS with EasySep Mouse Neutrophil Enrichment Kit (Stem cell). Normal neutrophils were isolated from tumor free (TF) heathy control mice by the same methods. Total RNA was extracted by TRIZOL (Invitrogen) for RNAseq and comparison.

多形核髓系来源抑制细胞（Polymorphonuclear myeloid-derived suppressor cell，PMN-MDSC），又称病理性活化中性粒细胞，是肿瘤微环境（tumor microenvironment，TME）的关键组成部分，在肿瘤进展与治疗抵抗中发挥重要作用。本研究对B16荷瘤小鼠来源的PMN-MDSC与无瘤小鼠来源的中性粒细胞的转录组开展了比较分析。实验设计概述：将B16-F10黑色素瘤细胞皮下接种于野生型C57/BL6小鼠体内。接种后第15天，通过AutoMACS结合EasySep小鼠中性粒细胞富集试剂盒（Stem cell）从荷瘤（tumor bearing，TB）小鼠的脾脏中分离得到PMN-MDSC；采用相同方法从无瘤（tumor free，TF）健康对照小鼠体内分离得到正常中性粒细胞。采用TRIZOL（Invitrogen）提取总RNA，用于转录组测序及比较分析。