ena-DATASET-NIBR-01-10-2017-21:05:55:926-108 - samples

We performed RNA-seq on polyA-enriched mRNA isolated from the original liver biopsy tissue (Liver tissue), primary liver cells (PLC), hepatocyte-like cells (HLCs) differentiated from induced pluripotent stem cells (iPSCs), and iPSCs. RNA libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Preparation protocol (ref. RS-122-2101, Illumina, San Diego CA, US) and sequenced using the Illumina HiSeq2500 platform following the manufacturer’s protocol. Samples were sequenced in paired-end mode to a length of 2x76 base pairs. Images from the instrument were processed using the manufacturer’s software to generate FASTQ sequence files.EGA dataset EGAD00001003770

本研究对从原始肝活检组织（Liver tissue）、原代肝细胞（PLC）、诱导多能干细胞（iPSCs）分化得到的类肝细胞（HLCs）以及诱导多能干细胞（iPSCs）中分离的聚腺苷酸化富集信使RNA（polyA-enriched mRNA）进行了RNA测序。RNA文库构建采用Illumina TruSeq链特异性mRNA样本制备方案（货号RS-122-2101，Illumina公司，美国加利福尼亚州圣地亚哥），并依照厂商官方操作流程，在Illumina HiSeq2500测序平台上完成测序。测序采用双端测序模式，读取长度为2×76碱基对。测序仪产出的图像通过厂商配套软件进行处理，最终生成FASTQ格式测序文件。该数据集为欧洲基因组-表型档案库（European Genome-phenome Archive, EGA）收录的EGAD00001003770数据集。