Genome-wide profiling of H3K4me3, H3K27me3 H3K27me1 and 5mC enrichment and mRNA levels in young leaves of Arabis alpina. Arabis alpina

Chromatin, DNA and RNA were extracted from young A. alpina Pajares primary stem leaves. Chromatin and DNA methylation immunoprecipitation experiments were performed using commercially available antibodies and analyzed by Illumina sequencing (ChIP-seq and MeDIP-seq). Transcriptome data were generated by RNA-seq. Overall design: ChIP-seq analysis of H3K4me3, H3K27me3 and H3K27me1 enrichment profiles and MeDIP-seq analysis of 5mC enrichment profiles in 2 biological replicates. RNA-seq analysis of mRNA levels in 1 biological replicate.

本研究从幼嫩的高山南芥（Arabis alpina）Pajares株系的主茎叶片中提取染色质、DNA与RNA。采用商品化抗体开展染色质免疫沉淀与甲基化DNA免疫沉淀实验，并通过Illumina测序结合染色质免疫沉淀测序（ChIP-seq）、甲基化DNA免疫沉淀测序（MeDIP-seq）完成数据分析。转录组数据由RNA测序（RNA-seq）生成。
整体实验设计：针对2个生物学重复样本，开展H3K4me3、H3K27me3及H3K27me1富集图谱的ChIP-seq分析，以及5mC富集图谱的MeDIP-seq分析；针对1个生物学重复样本，开展mRNA表达水平的RNA-seq分析。