Gene expression profiling of TH-MYCN mouse neuroblastoma sphere cells and their parental primary tumor cells

We performed microarray gene expression profiling to identify genes essential for the growth and tumorigenicity of mouse neuroblastoma stem-like cells Affymetrix microarray assays were performed according to the manufacturer's directions on total RNA isolated from three independent lines of mouse neuroblastoma sphere cells and their parental primary tumor cells

本研究开展基因芯片表达谱分析，旨在筛选对小鼠神经母细胞瘤干细胞样细胞的生长及致瘤性至关重要的基因。本研究采用Affymetrix基因芯片（Affymetrix microarray），严格按照制造商的操作说明，对从三株独立的小鼠神经母细胞瘤神经球细胞及其亲本原代肿瘤细胞中分离得到的总RNA进行了检测实验。