A new knockdown resistance (kdr) mutation, F1534L, in the voltage-gated sodium channel of Aedes aegypti, co-occurring with F1534C, S989P and V1016G

Aedes aegypti collected from Bengaluru were subjected to insecticide susceptibility tests with DDT, deltamethrin and permethrin. The DNA sequencing of partial domain II, III and IV of the voltage-gated sodium channel was performed to screen kdr mutations present in the population and PCR-based assays were developed for their detection. Genotyping of kdr mutations was done using PCR-based assays, allelic frequencies were determined, and tests of genetic association of kdr mutations with the insecticide resistance phenotype were performed. (MapVEu VBP0000681)

从班加罗尔（Bengaluru）采集的埃及伊蚊（Aedes aegypti），采用滴滴涕（DDT）、溴氰菊酯（deltamethrin）与氯菊酯（permethrin）开展杀虫剂敏感性试验。对电压门控钠通道（voltage-gated sodium channel）的部分Ⅱ、Ⅲ、Ⅳ结构域进行DNA测序，以筛选种群中携带的kdr突变（knockdown resistance mutations）；并开发基于聚合酶链式反应（Polymerase Chain Reaction，PCR）的检测方法用于该类突变的鉴定。通过该PCR检测方法完成kdr突变的基因分型，测定等位基因频率，并开展kdr突变与杀虫剂抗性表型的遗传关联分析。（MapVEu VBP0000681）