Systematic analysis of pfl ZTP riboswitch folding using Transcription Elongation Complex display (TECdisplay). Clostridium beijerinckii

RNA begins to fold as it emerges from an RNA polymerase (RNAP) during transcription. The physical coupling of nascent RNA to RNAP enables a functional interplay between transcription and RNA folding: the 5' to 3' direction of transcription restricts what RNA structures can fold, and conversely, nascent RNA can control transcription by interacting directly with RNAP. To investigate the mechanisms of cotranscriptional RNA structure formation we developed a versatile platform, called Transcription Elongation Complex display (TECdisplay), that systematically measures the activity of RNA sequence variants by quantitatively partitioning a DNA sequence library based on the function of cotranscriptionally displayed RNA. This BioProject contains raw sequence reads from TECdisplay experiments that characterize the mechanisms of Clostridium beijerinckii pfl ZTP riboswitch folding by determining the transcription antitermination activity of 65,536 pfl ZTP riboswitch variants.

RNA在转录过程中从RNA聚合酶（RNA polymerase, RNAP）中延伸出时便开始折叠。新生RNA与RNAP的物理偶联使得转录与RNA折叠之间形成功能性互作：转录的5'→3'方向会限制RNA可折叠的结构类型，反之，新生RNA也可通过直接与RNAP相互作用调控转录。为探究共转录RNA结构形成的机制，我们开发了一款名为转录延伸复合物展示（Transcription Elongation Complex display, TECdisplay）的通用实验平台，该平台可基于共转录展示的RNA的功能，通过定量划分DNA序列文库的方式，系统性地测定RNA序列变体的活性。本生物项目（BioProject）包含TECdisplay实验的原始测序读段，这些实验通过测定65536个pfl ZTP核糖开关变体的转录抗终止活性，解析了拜氏梭菌pfl ZTP核糖开关的折叠机制。