HK-2 cells: control and Substance P (SP)-treated

NK-1R axis in HK-2 cells by treated HK-2-overexpressed NK-1R cells with or without SP. Overall design: S) under 5% CO2 and 37 °C conditions. For experiment,we added 20 µM SP or PBS to NK-1R-overexpressed HK-2 cells for a 48-h incubation period, followed by harvesting for RNA isolation using Trizol reagent.

本数据集针对HK-2细胞中的神经激肽1受体（Neurokinin-1 Receptor，NK-1R）信号轴展开研究，实验采用过表达NK-1R的HK-2细胞，分别经或未接受P物质（Substance P，SP）处理。实验设计：细胞于5%CO₂、37℃条件下培养。实验流程：向过表达NK-1R的HK-2细胞中加入20 μM SP或磷酸盐缓冲液（Phosphate Buffered Saline，PBS），孵育48小时后收集细胞，使用Trizol试剂进行RNA分离提取。