Transcript profile analysis suggests marker genes to distinguish between Phase 0 and Phase 1 responses of Arabidopsis to salt stress. Transcript profile analysis suggests marker genes to distinguish between Phase 0 and Phase 1 responses of Arabidopsis to salt stress

Most physiological and molecular mechanisms of salinity stress are researched based on salt shock conditions. However, salt shock doesn’t occur in agricultural practice or natural ecosystem. In the fields, salts accumulate gradually through high salt or sodium irrigation water and poor managements that allow ground water to rise to soil surface. Therefore, it is more reasonable to research salinity stress in a mild way (stepwise salt addition). The objective of this study is to select marker genes to differentiate between salt shock (Phase 0) and salt stress (Phase 1). Three replicates were used for all RNA-Seq experiments conducted on control, Phase 0 and Phase 1 samples in Arabidopsis thaliana. Phase 0 samples (rosette leaves) were harvested 1 hour later from 105 mM NaCl salt shock treatment; Phase 1 samples (rosette leaves) were harvested 2 days later after reaching 90 mM NaCl by a stepwise addition (15 mM NaCl per day). Overall design: Three replicates were used for all RNA-Seq experiments conducted on control, Phase 0 and Phase 1 samples in Arabidopsis thaliana.

当前针对盐胁迫的生理与分子机制研究，大多基于盐骤击（salt shock）条件开展。但在农业生产实践与自然生态系统中，盐骤击现象实际并不存在。农田环境中，盐分往往通过高盐/含钠灌溉水，加之因管理不当导致地下水上升至地表，逐渐累积。因此，采用温和的逐步增盐法开展盐胁迫研究更为合理。本研究的目标为筛选可区分盐骤击（salt shock）阶段0（Phase 0）与渐进式盐胁迫阶段1（Phase 1）的标记基因。本研究针对拟南芥（Arabidopsis thaliana）的对照组、阶段0（Phase 0）组与阶段1（Phase 1）组样本开展RNA测序（RNA-Seq）实验，所有实验均设置3次生物学重复。阶段0（Phase 0）组样本为莲座叶（rosette leaves），经105 mM NaCl盐骤击处理1小时后采集；阶段1（Phase 1）组样本同为莲座叶（rosette leaves），通过每日添加15 mM NaCl的逐步增盐方式，使培养液NaCl浓度达到90 mM后继续培养2天采集。实验整体设计：针对拟南芥（Arabidopsis thaliana）的对照组、阶段0（Phase 0）组与阶段1（Phase 1）组样本开展的所有RNA测序（RNA-Seq）实验，均设置3次生物学重复。