Novel eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence

SourceData_Fig2f: Relative expression of XopD, PR1, PR2, OSCA1.1 and HYL1 after XopD was induced by β-estradiol in Arabidopsis seedlings, relative to their expression in DMSO-treated seedlings. SourceData_Fig3b: Disease index scores (0, no symptoms; 0.5 to 1.5, weak chlorosis; 2 to 3, strong chlorosis) from n=40 each of osca1-1 or Col-0 leaves infected with Xcc* from 8 individual plants, at 7 DPI. SourceData_Fig3c: Bacterial population density in n=23 osca1-1 or n=24 Col-0 leaves infected with Xcc* from 8 different plants, at 7 DPI. SourceData_Fig3e: Relative expression of OSCA1.1 in Col-0 leaves infiltrated with Xcc∆xopD*dTALE#1 (dTALE#1) and Xcc∆xopD*dTALE#2 (dTALE#2), relative to its expression in Xcc∆xopD*vec2 (vector) infiltrated leaves, at 1 DPI. SourceData_Fig3h: Bacterial population density in Col-0 leaves inoculated with Xcc∆xopD* (-XopD) and Xcc* (+XopD) strains that deliver empty vector (vector), dTALE#1 or dTALE#2, at 7 DPI. SourceData_Fig4b: RT-qPCR analysis of RD29A expression in nuc+XopD relative to its expression in nuc-XopD. SourceData_Fig4c: Relative abundances of miR159a in nuc-XopD and nuc+XopD compared to its abundance in total nuclei of mock-inoculated leaves. SourceData_Fig4e: The water loss rate of detached Xcc* (+XopD) and Xcc∆xopD* (-XopD) infected leaves at 7 DPI. Data are presented as mean values +/- SD (error bars) from n=8 leaves from 4 different plants. SourceData_Fig4f: Bacterial population density in symptomatic leaf tissues inoculated with Xcc* (+XopD) and Xcc∆xopD* (-XopD) at 7 DPI. SourceData_ED_Fig2c: Bacterial population density in n=12 leaves of the eINTACT reporter line inoculated with Xcc*AvrBs3 and n=12 wild-type Col-0 leaves inoculated with Xcc*vec1, at 7 DPI. SourceData_ED_Fig4: RT-qPCR analysis of relative expression of NRPD1A, DDM1, HYL1, DWA1 and OSCA1.1 in Xcc∆xopD*AvrBs3 (-XopD) and Xcc*AvrBs3 (+XopD) inoculated leaves of the eINTACT reporter line at 5 DPI. SourceData_ED_Fig6c: Disease index scores (0, no symptoms; 0.5 to 1.5, weak chlorosis; 2 to 3, strong chlorosis) from n=30 each of Col-0, osca1-1 or Pro35S:OSCA1.1/osca1-1 leaves infected with Xcc* from 8 individual plants at 7 DPI. SourceData_ED_Fig6d: Bacterial population density in n=12 each of Col-0, osca1-1 or Pro35S:OSCA1.1/osca1-1 leaves infected with Xcc* from 4 different plants at 7 DPI. SourceData_ED_Fig8: ABA levels in Xcc*AvrBs3 and XccΔxopD*AvrBs3 infected leaves, at 5 DPI. SourceData_ED_Fig9: Bacterial population density in Arabidopsis leaves inoculated with Xcc∆xopD* or Xcc*, at 1, 3, 5 and 7 DPI.

SourceData_Fig2f: 以二甲基亚砜（DMSO）处理的拟南芥幼苗中各基因表达量为参照，经β-雌二醇（β-estradiol）诱导XopD表达后，拟南芥幼苗中XopD、PR1、PR2、OSCA1.1及HYL1的相对表达量。
SourceData_Fig3b: 病害指数评分（0代表无病症；0.5~1.5为轻度褪绿；2~3为重度褪绿），数据取自8株不同拟南芥植株的各40片经Xcc*侵染的osca1-1与Col-0叶片，检测时间为接种后7天（DPI，days post inoculation）。
SourceData_Fig3c: 取自8株不同拟南芥植株的23片osca1-1叶片与24片Col-0叶片（均经Xcc*侵染）中的细菌种群密度，检测时间为接种后7天（DPI，days post inoculation）。
SourceData_Fig3e: 以经Xcc∆xopD*vec2（空载体）真空浸润接种的Col-0叶片中OSCA1.1的表达量为参照，经Xcc∆xopD*dTALE#1（dTALE#1）与Xcc∆xopD*dTALE#2（dTALE#2）真空浸润接种的Col-0叶片中OSCA1.1的相对表达量，检测时间为接种后1天（DPI，days post inoculation）。
SourceData_Fig3h: 分别接种携带空载体、dTALE#1或dTALE#2的Xcc∆xopD*（-XopD，缺失XopD）与Xcc*（+XopD，携带XopD）菌株的Col-0叶片中的细菌种群密度，检测时间为接种后7天（DPI，days post inoculation）。
SourceData_Fig4b: 以nuc-XopD（不含XopD的细胞核组分）样本中RD29A的表达量为参照，对nuc+XopD（含XopD的细胞核组分）样本中RD29A的表达量进行实时定量荧光PCR（RT-qPCR）分析。
SourceData_Fig4c: 以空白接种叶片的总细胞核中miR159a的丰度为参照，对比nuc-XopD与nuc+XopD样本中miR159a的相对丰度。
SourceData_Fig4e: 经Xcc*（+XopD，携带XopD）与Xcc∆xopD*（-XopD，缺失XopD）菌株侵染的离体叶片在接种后7天（DPI，days post inoculation）的失水速率。数据以平均值±标准差（误差棒）形式呈现，取自4株不同拟南芥植株的各8片叶片。
SourceData_Fig4f: 分别接种Xcc*（+XopD，携带XopD）与Xcc∆xopD*（-XopD，缺失XopD）菌株并出现病症的叶片组织中的细菌种群密度，检测时间为接种后7天（DPI，days post inoculation）。
SourceData_ED_Fig2c: 分别接种Xcc*AvrBs3的eINTACT报告株系的12片叶片，与接种Xcc*vec1的野生型Col-0的12片叶片中的细菌种群密度，检测时间为接种后7天（DPI，days post inoculation）。
SourceData_ED_Fig4: 对eINTACT报告株系分别接种Xcc∆xopD*AvrBs3（-XopD，缺失XopD）与Xcc*AvrBs3（+XopD，携带XopD）的叶片中NRPD1A、DDM1、HYL1、DWA1及OSCA1.1的相对表达量进行实时定量荧光PCR（RT-qPCR）分析，检测时间为接种后5天（DPI，days post inoculation）。
SourceData_ED_Fig6c: 病害指数评分（0代表无病症；0.5~1.5为轻度褪绿；2~3为重度褪绿），数据取自8株不同拟南芥植株的各30片经Xcc*侵染的Col-0、osca1-1及Pro35S:OSCA1.1/osca1-1株系叶片，检测时间为接种后7天（DPI，days post inoculation）。
SourceData_ED_Fig6d: 取自4株不同拟南芥植株的各12片经Xcc*侵染的Col-0、osca1-1及Pro35S:OSCA1.1/osca1-1株系叶片中的细菌种群密度，检测时间为接种后7天（DPI，days post inoculation）。
SourceData_ED_Fig8: 经Xcc*AvrBs3与XccΔxopD*AvrBs3菌株侵染的叶片中的脱落酸（ABA，abscisic acid）水平，检测时间为接种后5天（DPI，days post inoculation）。
SourceData_ED_Fig9: 分别接种Xcc∆xopD*与Xcc*菌株的拟南芥叶片中的细菌种群密度，检测时间为接种后1、3、5及7天（DPI，days post inoculation）。