mRNA Breast Cancer Cell Line Profiles. Homo sapiens

mRNA breast cancer cell lines were profiled to study the function of hsa-mir-221 and hsa-mir-222. MCF7 cell lines were profiled after treatment with mir-221/222 mimics, and compared to profiles with transfection controls. Similarly, MDA-MB-231 cell lines were profiled after treatment with mir-221/222 inhibitors, and compared to profiles with transfection controls. Since ESR1 is a predicted target of mir-221/222 we also profiled MCF7 cell lines after disrupting ESR1 with an siRNA. Other breast cancer cell lines are provided because all cell lines were normalized together. Keywords: breast cancer, cell line, hsa-mir-221, hsa-mir-222, ESR1 Overall design: mRNA breast cancer cell line profiles with some samples in duplicate or triplicate. See summary for more information.

本数据集通过mRNA表达谱分析乳腺癌细胞系，以探究hsa-mir-221与hsa-mir-222的生物学功能。针对MCF7细胞系，本研究对经mir-221/222模拟物（mir-221/222 mimics）处理后的样本进行了mRNA表达谱检测，并与转染对照组（transfection controls）的表达谱数据进行对比分析；同理，本研究对经mir-221/222抑制剂（mir-221/222 inhibitors）处理后的MDA-MB-231细胞系开展了表达谱分析，并与转染对照组进行比对。鉴于ESR1被预测为mir-221/222的靶基因，本研究还通过小干扰RNA（siRNA，small interfering RNA）敲低ESR1后，对MCF7细胞系进行了表达谱检测。由于所有细胞系均统一进行了标准化处理，故一并提供了其余乳腺癌细胞系的表达谱数据。关键词：乳腺癌（breast cancer）、细胞系（cell line）、hsa-mir-221、hsa-mir-222、ESR1。实验设计概述：本数据集包含乳腺癌细胞系的mRNA表达谱数据，部分样本设置了两次或三次生物学重复，详细信息请参见数据集摘要。