Next Generation Sequencing Methylation Profiling in Skeletal Muscle from Lean and Obese Subjects [Microarray expression]. Homo sapiens

Obesity is a metabolic disease caused by environmental, genetic, and epigenetic factors. However, the epigenetic mechanisms of obesity are incompletely understood. The aim of our study was to identify skeletal muscle DNA methylation patterns in combination with transcriptomic changes in obesity. Overall design: Muscle biopsies were obtained basally from lean (n=10) and obese (n=10) participants in combination with euglycemic hyperinsulinemic clamps to assess insulin sensitivity. We performed microarray (SurePrint G3 Human Gene Expression 8x60K v2) analysis on RNA isolated from vastus lateralis muscle biopsies. This represents the expression profiling component only.

肥胖是一种由环境、遗传以及表观遗传（epigenetic）因素共同诱发的代谢性疾病。然而目前针对肥胖的表观遗传调控机制仍未完全阐明。
本研究旨在探究肥胖状态下骨骼肌（skeletal muscle）的DNA甲基化（DNA methylation）模式，并结合转录组学（transcriptomic）变化开展联合分析。
整体实验设计：本研究从10名瘦体型受试者（n=10）与10名肥胖受试者（n=10）体内采集基础状态下的肌肉活检样本，并结合正常血糖高胰岛素钳夹试验（euglycemic hyperinsulinemic clamps）评估受试者的胰岛素敏感性。
我们针对从股外侧肌（vastus lateralis）活检样本中提取的RNA，采用SurePrint G3 Human Gene Expression 8x60K v2芯片进行了微阵列（microarray）分析。
本部分仅涵盖表达谱分析相关内容。