Superfrogs in the city: 150-year impact of urbanization and agriculture on the European Common Frog (data)

Data on body size (SVL), fluctuating asymmetry (CFA), and stable isotopes (δ15N, δ13C) from museum specimens (n=158) of the European Common Frog, Rana temporaria, all originating from the Berlin-Brandenburg area, Germany, and related land-use variables (QGIS; 1km buffer) in order to test: (i) if specimens have changed over the last 150 years (1868-2018), and (ii) if changes could be attributed to increasing urbanization and agricultural intensity. SVL was measured with a digital caliper. For CFA calculations, measurements of femora, tibio-fibulae, humeri, and radio-ulnae were taken from µCT-volumetric reconstructions (Phoenix|X-ray nanotom). For determining stable isotopes, thigh muscle tissue from the samples was dried and analyzed with a isotope ratio mass spectrometer (THERMO/Finnigan MAT V).

本数据集涵盖采自德国柏林-勃兰登堡地区的158份欧洲林蛙（Rana temporaria）馆藏标本的体型数据（吻肛长，SVL）、波动不对称性（CFA）以及稳定同位素（δ¹⁵N、δ¹³C）数据，同时配套了基于QGIS生成的1km缓冲区相关土地利用变量数据，用于验证两项研究假设：(i) 1868年至2018年的近150年间，该物种标本的相关指标是否发生变化；(ii) 此类变化是否可归因于城市化水平提升与农业集约化程度加剧。吻肛长（SVL）采用数显卡尺进行测量。波动不对称性（CFA）的计算所需的股骨、胫腓骨、肱骨及桡尺骨测量数据，来源于µCT体积重建（Phoenix|X-ray nanotom）扫描结果。稳定同位素分析环节，研究人员对样本大腿肌肉组织进行干燥处理后，通过同位素比率质谱仪（THERMO/Finnigan MAT V）完成检测分析。