Mass spectrometry analysis of in vitro Tau ubiquitination by the UBA1/Hsp70/CHIP machinery

We aimed at characterizing which lysines on Tau were ubiquitinated by the UBA1/Hsp70/CHIP machinery. We also determined the ubiquitin linkages of the assembled Tau chains.

本研究旨在明确Tau蛋白（Tau）上的哪些赖氨酸残基可被UBA1/Hsp70/CHIP复合物介导泛素化，同时还确定了组装形成的Tau泛素链的泛素连接类型。