Regulation of the DNA damage response and melanoma susceptibility by NCOA3. Regulation of the DNA damage response and melanoma susceptibility by NCOA3

NCOA3 has important roles in melanoma as a potential diagnostic and susceptibility marker, and as a target for therapy Overall design: Anti NCOA3 shRNA vs control shRNA were stably transfected in C8161.9 cell lines to demonstrate differentially expressed genes in cDNA microarray run in duplicate.

核受体辅激活因子3（NCOA3）在黑色素瘤中具有重要作用，可作为潜在的诊断标志物、易感标志物及治疗靶点。实验设计：将靶向NCOA3的短发夹RNA（short hairpin RNA，shRNA）与对照短发夹RNA稳定转染至C8161.9细胞系，通过两次重复运行的cDNA微阵列（cDNA microarray）实验，鉴定差异表达基因。