Gene expression profiling of CD34+ subsets in Multiple Myeloma and healthy individuals. Homo sapiens

Multiple myeloma (MM) is a clonal plasma cell disorder frequently accompanied by hematopoietic impairment. Genomic profiling of distinct HSPC subsets revealed a consistent deregulation of signaling cascades, including TGF beta signaling, p38MAPK signaling and pathways involved in cytoskeletal organization, migration, adhesion and cell cycle regulation in MM patients. Overall design: CD34+ subsets of 7 patients with Multiple Myeloma and 5 healthy volunteers were analysed by means of gene expression profiling with the Affymetrix HU-133A 2.0 array

多发性骨髓瘤（Multiple myeloma, MM）是一类克隆性浆细胞疾病，常伴随造血功能损伤。对不同造血干祖细胞（hematopoietic stem and progenitor cells, HSPC）亚群进行基因组分析后发现，多发性骨髓瘤患者体内存在信号级联反应的持续失调，涉及转化生长因子β（TGF-β）信号通路、p38丝裂原活化蛋白激酶（p38MAPK）信号通路，以及参与细胞骨架组织、细胞迁移、细胞黏附与细胞周期调控的多条通路。本研究的实验设计如下：采集7名多发性骨髓瘤患者与5名健康志愿者的CD34阳性细胞亚群，采用Affymetrix HU-133A 2.0基因芯片开展基因表达谱分析。