Data from: Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil

The chlorothalonil is a widely used fungicide while the contamination of
soil and water environments by this chemical causes potential threats to
the biodiversity. Given the metabolic potential of soil microorganisms,
there is a need for developing microbiological approaches to degrade
persistent compounds, such as chlorothalonil, in the contaminated sites.
Here in this study, we isolated a bacterial strain (namely, BJ1) capable
of degrading chlorothalonil from a chlorothalonil-contaminated farmland
soil in the Shandong Province, China. Using 16S rDNA gene sequencing,
morphological and biological characteristics, we identified the strain BJ1
as Stenotrophomonas acidaminiphila. The strain BJ1 utilizes chlorothalonil
as a sole carbon source. At initial concentrations of 50, 100, 200, 300 mg
l-1, it degraded 91.5%, 89.4%, 86.5 and 83.5% of chlorothalonil after 96 h
of inoculation under optimum conditions (30 °C and pH 7.0), respectively.
Two metabolites, methyl-2, 5, 6-trichloro-3-cyano-4-methoxy-benzoate and
methyl-3- cyano-2, 4, 5, 6-tetrachlorobenzoate, were detected and
identified based on HPLC-MS analysis, which suggests that the strain BJ1
metabolized chlorothalonil through the hydroxylation of chloro-group and
hydration of cyano-group. The results of this study highlight the great
potential for this bacterium to be used in chlorothalonil pollution
remediation.

百菌清（chlorothalonil）是一类应用广泛的杀菌剂，其对土壤与水环境造成的污染会对生物多样性构成潜在威胁。鉴于土壤微生物具备代谢潜力，开发微生物修复技术以降解污染场地中百菌清等持久性污染物具有重要现实意义。本研究从中国山东省某受百菌清污染的农田土壤中，分离得到一株可降解百菌清的细菌菌株BJ1。通过16S rDNA基因测序、形态学及生物学特性分析，将菌株BJ1鉴定为嗜氨基酸寡养单胞菌（Stenotrophomonas acidaminiphila）。该菌株以百菌清作为唯一碳源进行生长代谢。在最优培养条件（30℃、pH 7.0）下接种96小时后，当初始百菌清浓度分别为50、100、200、300 mg/L时，菌株对其降解率依次为91.5%、89.4%、86.5%和83.5%。通过高效液相色谱-质谱联用法（HPLC-MS）检测并鉴定出两种代谢产物：2,5,6-三氯-3-氰基-4-甲氧基苯甲酸甲酯与3-氰基-2,4,5,6-四氯苯甲酸甲酯，由此推测菌株BJ1通过氯基羟基化与氰基水合作用完成百菌清的代谢过程。本研究结果表明，该菌株在百菌清污染修复领域具备极高应用潜力。