Additional file 1 of Chalkiness and premature controlled by energy homeostasis in OsNAC02 Ko-mutant during vegetative endosperm development

Additional file 1: Figure S1. The mutant tags of target sites and mutants detection by PCR sequencing. Figure S2. The yield characters of T2 N2 /N3 mut (95 DAG) in field (95 DAG in 2021, 125 DAG in 2020). Figure S3. The homologous clusters of OsNAC family genes by phylogenetic analysis. Figure S4. The homologous analysis of NAC in plant. The Gene ID are according to the database RAP (rapdb.dna.affrc.go.jp), and (OsNAC02, Os02g0594800), (OsNAC06, Os06g0267500) in NCBI of homologous analysis. Figure S5. The ABRE/ G-box motif locations on the promoter regions of OsNAC02 regulated genes. Figure S6. The chalky phenotype of N2/N3 mut seeds (T2) in 2021. Figure S7. The histological analysis during endosperm development. Figure S8. The PCA clusters based on RNA-seq analysis. Figure S9. The heatmap diagram of DEGs in N2/N3 versus WT. Figure S10. The genes of 39 ECs in Sucrose and Starch metabolic pathway in Mapman of N2 vs WT seeds (3DAP) are verified by qRT-PCR. Figure S11. The antagonism between energy storage and development requirement under stress in Ko-Osnac02 mutant.

附加文件1：
图S1：靶位点突变标签及PCR测序验证的突变体鉴定
图S2：T2代N2/N3突变体（95 DAG）的田间产量性状（2021年为95 DAG，2020年为125 DAG）
图S3：基于系统发育分析的OsNAC家族基因同源聚类
图S4：植物NAC基因的同源性分析，基因ID参考自RAP数据库（rapdb.dna.affrc.go.jp），同源分析所用的(OsNAC02, Os02g0594800)、(OsNAC06, Os06g0267500)序列来自NCBI数据库
图S5：OsNAC02调控基因启动子区域的ABRE/G-box基序定位
图S6：2021年N2/N3突变体T2代种子的垩白表型
图S7：胚乳发育过程的组织学分析
图S8：基于RNA-seq分析的PCA聚类结果
图S9：N2/N3与野生型（Wild Type, WT）间差异表达基因（Differentially Expressed Genes, DEGs）的热图
图S10：Mapman注释的N2与野生型种子（3DAP，即开花后3天）蔗糖和淀粉代谢通路中的39个酶编码基因（Enzyme Commission, ECs）经qRT-PCR验证
图S11：Ko-Osnac02突变体中能量储存与逆境胁迫下发育需求的拮抗作用