Single cell gene expression profiling of endothelial cells from the aorta of wild type and amotl2 ec-/ec- mice

Deletion of AmotL2 in endothelial cells has a pronounced effect on nuclear shape and subcellular positioning. Difference in the phenotype of WT and AmotL2 KO endothelial cells is elucidated by scRNA-seq on both populations. Aortae from 6 mice from WT and 6 AmotL2 KO mice were dissociated. Single cell suspension was then depleted of Cd45+ cells with magnetic bead separation and Endothelial cells positively sorted out as Cd31+, CD45- cells. CD31-, CD45- cells were sorted as well as control. The dataset is from two separate preparations of cells (12 mice in total).

内皮细胞中AmotL2基因的缺失会对细胞核形态与亚细胞定位产生显著影响。通过对两组细胞开展单细胞RNA测序（single-cell RNA sequencing, scRNA-seq），可阐明野生型（WT, Wild Type）与AmotL2基因敲除（KO, Knockout）内皮细胞的表型差异。分别从6只野生型小鼠和6只AmotL2基因敲除小鼠体内分离主动脉并进行组织解离，制备得到单细胞悬液；随后通过磁珠分选去除悬液中的CD45阳性细胞，并将CD31阳性、CD45阴性的细胞作为内皮细胞进行正向分选，同时分选CD31阴性、CD45阴性的细胞作为对照。本数据集来源于两次独立的细胞制备实验，共使用12只小鼠。