Loss of early induction of CD11c positive microglia in lipopolysaccharide-induced maternal immune activation model.

The concept of maternal immune activation (MIA) has been expanded to chorioamnionitis-associated intrauterine inflammation. We have previously reported that autism-like behavioral deficits were detected in the lipopolysaccharide (LPS)-induced MIA mouse model. The pregnant mice were injected with PBS (control) or LPS. The microglia were isolated from neonatal mice brains at postnatal day 3 and RNA Seq analyzed transcriptomic profiles.

母体免疫激活（Maternal Immune Activation, MIA）的概念已拓展至绒毛膜羊膜炎相关宫内炎症领域。本课题组此前曾报道，在脂多糖（Lipopolysaccharide, LPS）诱导的MIA小鼠模型中可检测到类自闭症行为缺陷。实验中，孕鼠分别接受磷酸盐缓冲盐水（Phosphate Buffered Saline, PBS，对照组）或LPS注射。研究人员于出生后第3天从新生小鼠脑组织中分离小胶质细胞，并通过RNA测序（RNA Sequencing, RNA-seq）分析其转录组表达谱。