Klebsiella pneumoniae Genome sequencing

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an emerging nosocomial pathogen of major concern. We investigated the clonal diversity and dissemination of CRKP in Malaysia between January 2022 and December 2023. A total of 147 CRKP isolates were collected from diverse clinical settings, all resistant to at least one carbapenem, with 124 confirmed as carbapenemase producers. ERIC-PCR genotyping revealed 24 clusters across multiple wards. Twelve representative isolates, selected based on epidemiological data and clustering, underwent whole-genome sequencing (WGS). Genomic analyses confirmed clonal dissemination of ST17 as the dominant CRKP lineage and identified bla_NDM as the key carbapenemase gene in all sequenced isolates. This integrated approach combining phenotypic, molecular, and genomic data provides insights into resistance dynamics, transmission, and supports effective infection control strategies.

耐碳青霉烯类肺炎克雷伯菌（Carbapenem-resistant Klebsiella pneumoniae, CRKP）是一类备受关注的新发院内病原菌。本研究于2022年1月至2023年12月期间，针对马来西亚境内的CRKP克隆多样性与传播流行特征展开了调查。本研究共从各类临床诊疗场景中收集到147株CRKP分离株，所有菌株均对至少一种碳青霉烯类抗生素耐药，其中124株经确认可产碳青霉烯酶。经ERIC-PCR基因分型检测，多个病房中共检出24个克隆簇。研究人员基于流行病学数据与克隆簇分组，选取12株代表性分离株开展全基因组测序（Whole-Genome Sequencing, WGS）。基因组分析结果证实，ST17型（Sequence Type 17）作为优势克隆谱系在CRKP中发生克隆传播，且所有测序分离株的关键碳青霉烯酶基因均为bla_NDM。这种整合表型、分子与基因组学数据的一体化研究策略，不仅为阐明耐药性动态变化与传播机制提供了新见解，同时可为制定高效感染防控策略提供有力支撑。