Data for: Phenotypic adaptation to temperature in the mosquito vector, Aedes aegypti

Most models exploring the effects of climate change on mosquito-borne disease ignore thermal adaptation. However, if local adaptation leads to changes in mosquito thermal responses, ‘one size fits all’ models could fail to capture current variation between populations and future adaptive responses to changes in temperature. Here we assess phenotypic adaptation to temperature in Aedes aegypti, the primary vector of dengue, Zika, and chikungunya viruses. First, to explore whether there is any difference in existing thermal response of mosquitoes between populations we used a thermal knockdown assay to examine five populations of Ae. aegypti collected from climatically diverse locations in Mexico, together with a longstanding laboratory strain. We identified significant phenotypic variation in thermal tolerance between populations. Next, to explore whether such variation can be generated by differences in temperature we conducted an experimental passage study by establishing six replicate ..., Mosquito Collection
Aedes aegypti mosquitoes were collected from the field using ovitraps in five different locations in Mexico (Cabo San Lucas, Acapulco, Monterrey, Ciudad Juárez, and Jojutla) and compared to a standard laboratory population (Rockefeller strain) maintained at Penn State University. The field locations were chosen to capture a gradient of the landscape and climate, driven primarily by variation in altitude. Populations were founded with sufficient viable eggs collected from multiple ovitraps across the cities to yield at least 100 adult mosquitoes in the F1 laboratory generation. Mosquitoes were reared for a generation (F2) in standard laboratory conditions (27°C, 80% humidity, 12:12hr photoperiod, 0.60 mg of bovine liver powder per 600 larvae and ad libitum access to 10% sugar solution for the adults) prior to experimentation to remove the influence of any maternal effects. Two populations, Jojutla and Juárez, were reared for an additional generation (F3) to ensure a l..., , # Associated data for \"Phenotypic adaptation to temperature in the mosquito vector, *Aedes aegypti*\"

[https://doi.org/10.5061/dryad.z8w9ghxgc](https://doi.org/10.5061/dryad.z8w9ghxgc)

We used explored mosquito adaptation to temperature in the vector species *Aedes aegypti* through thermal knockdown of field mosquitoes and life history experiments of experimentally adapted mosquitoes. We used survival analysis and a Bayesian approach to analyze these data.

## Description of the data and file structure

There are three types of files found in this set, excel/csv files containing raw data, R code and text files (containing models for Bayesian models). There are three sets of data containing field mosquito knockdown data, experimental passage mosquito knockdown data, and life history data for experimental passage mosquitoes. Both knockdown data sets correspond to R code labeled knockdown models. Selection experiment data correspond to models using a Bayesian approach. Models for mosquito...

现有探究气候变化对蚊媒疾病影响的模型，大多忽略了热适应性这一关键因素。然而若本地适应性导致蚊子热响应特征发生改变，"一刀切"的模型将无法准确捕捉种群间的现存差异，以及未来种群对温度变化的适应性响应。
本研究针对登革热、寨卡病毒及基孔肯雅热的主要传播媒介埃及伊蚊（Aedes aegypti）的温度表型适应性展开评估。首先，为探究不同种群蚊子的现有热响应是否存在差异，我们采用热击倒实验（thermal knockdown assay），对从墨西哥不同气候区域采集的5个埃及伊蚊种群，以及一株长期传代的实验室品系进行了检测。我们发现不同种群间的热耐受表型存在显著差异。其次，为探究此类差异是否可由温度差异诱导产生，我们通过实验传代研究开展了验证：构建了6个重复实验组……，## 蚊虫采集
研究人员于墨西哥5个不同地点（卡波圣卢卡斯、阿卡普尔科、蒙特雷、华雷斯城和约胡特拉）利用诱卵器（ovitraps）采集野外埃及伊蚊，并与美国宾夕法尼亚州立大学保藏的标准实验室种群（洛克菲勒品系，Rockefeller strain）进行对照。野外采样点的选取旨在覆盖以海拔差异为主要驱动因素的景观与气候梯度。每个种群均通过采集城市内多个诱卵器获得的足量存活卵组建，确保F1代实验室饲养可获得至少100只成蚊。实验前，所有蚊虫均在标准实验室条件下饲养至F2代（温度27℃、相对湿度80%、光周期12:12、每600头幼虫饲喂0.60mg牛肝粉，成虫可自由取食10%蔗糖溶液），以消除母体效应的影响。其中约胡特拉种群与华雷斯城种群额外饲养至F3代，以确保……（原文未完成）
# 关联数据集：《蚊媒埃及伊蚊的温度表型适应性研究》
https://doi.org/10.5061/dryad.z8w9ghxgc
本研究通过野外蚊虫热击倒实验与实验适应性蚊虫的生活史实验，探究了媒介物种埃及伊蚊的温度适应性，并采用生存分析与贝叶斯方法对数据进行分析。
## 数据与文件结构说明
本数据集包含三类文件：存储原始数据的Excel/CSV文件、R代码文件，以及用于贝叶斯模型构建的文本文件。数据集包含三套核心数据：野外蚊虫热击倒数据、实验传代蚊虫热击倒数据，以及实验传代蚊虫的生活史数据。两套热击倒数据均对应标注为"knockdown models"的R代码脚本。选择实验数据对应采用贝叶斯方法构建的模型。蚊虫模型的相关内容……（原文未完成）